Yesterday we tested antibiotics and oils to see how they were effective against our bacteria.
Antibiotics:
1. Penicillin (P10): no effect
2. Erythromycin (E15): no effect                                                           2
3. Chlomiphenic (C30): Effective- 1.5cm diameter                         1   5   3
4. Novoblociv (NB30): no effect                                                           4
5. Augmentin: no effect

Oils:
1.Clove: somewhat effective-1cm diameter
2. Cinnamon: most effective- 1.5cm diameter                                  2
3. Thyme: 2nd most effective-1.2cm diameter                            1   5    3
4. Eucalypte: least effective-0.8cm diameter                                    4
5. Ginger: not effective

Yesterday we also tested for food purity.  We created 4 wells in a petri dish and added extract and albumin to them.
1. Hamburger extract
2. Goat Anti-horse Albumin                         2          3
3. Goat Anti-bovine Albumin                             1
4. Goat Anti-swine Albumin                               4
After leaving the dish in room temperature overnight, we observed a reaction between the hamburger extract and Anti-bovine Albumin.  The reaction is the white semicircular streak seen below between 1 and 3:

Next we tested a sample to determine if HIV was present. First, we added 50ul of the purified antigen into 9 wells and waited 5 minutes.  Then we washed the unbound antigen out of the wells.  After that we added 50ul of the positive control to the three "+" wells, and 50ul of the negative control to the three "-" wells.  Then we transfered 50ul of serum sample to the 3 remaining wells.  After 5 more minutes, we washed the wells out again.  Then we added 50ul of secondary antibody into all of the wells.  After 5 more minutes, we washed the unbound antibody out of the wells.  We then transfered 50ul of enzyme substrate into each of the 9 wells and observed changes in color. 

#microbiologylovers!