Antibiotics:
1. Penicillin (P10): no effect
2. Erythromycin (E15): no effect 2
3. Chlomiphenic (C30): Effective- 1.5cm diameter 1 5 3
4. Novoblociv (NB30): no effect 4
5. Augmentin: no effect
Oils:
1.Clove: somewhat effective-1cm diameter
2. Cinnamon: most effective- 1.5cm diameter 2
3. Thyme: 2nd most effective-1.2cm diameter 1 5 3
4. Eucalypte: least effective-0.8cm diameter 4
5. Ginger: not effective
Yesterday we also tested for food purity. We created 4 wells in a petri dish and added extract and albumin to them.
1. Hamburger extract
2. Goat Anti-horse Albumin 2 3
3. Goat Anti-bovine Albumin 1
4. Goat Anti-swine Albumin 4
After leaving the dish in room temperature overnight, we observed a reaction between the hamburger extract and Anti-bovine Albumin. The reaction is the white semicircular streak seen below between 1 and 3:
Next we tested a sample to determine if HIV was present. First, we added 50ul of the purified antigen into 9 wells and waited 5 minutes. Then we washed the unbound antigen out of the wells. After that we added 50ul of the positive control to the three "+" wells, and 50ul of the negative control to the three "-" wells. Then we transfered 50ul of serum sample to the 3 remaining wells. After 5 more minutes, we washed the wells out again. Then we added 50ul of secondary antibody into all of the wells. After 5 more minutes, we washed the unbound antibody out of the wells. We then transfered 50ul of enzyme substrate into each of the 9 wells and observed changes in color.
#microbiologylovers!
